Fig. 4

Histo-patholological evaluation of genital skin biopsies: immune-staining for commercially available ready to use mouse monoclonal antibody against Estrogen Receptor (ER) α and β. Upper row (a and b): Hormonally treated group (a) showed weak ERα expression (+) that was localized to the stratum basale, in contrast to the ‘untreated’ group (b) that showed no detectable expression (0). (original magnification × 400). Lower row (c and d): ERβ immunoreactivity was strongly expressed in the keratinocytes of the stratum basale and stratum spinosum (+++) in the treated group (c); while in untreated group (d), there was moderate expression (++). (A&B: ERβ, immunoperoxidase; original magnification × 400)