Fig. 3

Application of the physical dissector method, in which micrographs in the same fields of view (a, b) are taken from two parallel, adjacent thin sections separated by a distance of 5 mm. Upper and right lines of unbiased counting frames represent the inclusion lines; the lower and left lines, including the extensions, are exclusion lines. Any neuron nucleolus reaching the inclusion lines was excluded, and nucleolus profiles hitting the inclusion lines and located inside the frame were counted as dissector particles unless their profiles were extended up to the lookup section. The number of neurons from the two dissectors occurs in a volume given by the product of the counting frame area and the distance between the sections. The numerical density of the neurons is calculated as NvGN = ΣQ^-GN/txA. In this application, the nucleoli marked with “1, 3, 6, 8” are dissector particles in a, which disappeared in b. The nucleoli marked “2, 4, 5, 7” are not dissector particles in a, which disappeared in b (H&E, 400, LM)